The alcoholic fermentation process was carried out in November inside facilities at the ISS that mimicked lunar gravity, the two firms said in a joint statement, dated Tuesday.
The mash was returned to Earth in February and was refined into 116 milliliters of sake in March in Japan.
The drink was packaged in a 100-ml bottle and went to an anonymous buyer for 110 million yen ($686,000).
The remaining 16 ml allowed for taste tests.
“It offers a pronounced acidity. It has a well-balanced and robust sake flavor,” a Dassai spokesperson told Agence France-Presse.
Proceeds from the sale will be donated to support Japan’s space development efforts, the two firms said.
“This result demonstrates experimentally that sake production is feasible even under lunar-gravity conditions using a process comparable to that on Earth,” the firms said.
Dassai says it wants to build a sake brewery on the moon by 2050 to “improve quality of life for future lunar habitation.”