The annual "Walk for Life" will take place on Sunday, 23 February.
Organized by the Council of the Laity of the Philippines, the event will start at Rizal Park and end at the Manila Cathedral.
"Let's come together to celebrate the gift of life and bring the message of hope to the world," the organization said.
"Let's walk with purpose, stand for life, and be a beacon of hope," it added.
Participants are asked to assemble at 4:00 a.m. at Lawn C, Flower Clock Area of Rizal Park.
The two-kilometer walk will begin at 4:30 a.m., proceeding from Maria Orosa Street, then Muralla Street, and finally to Sto. Tomas Avenue.
Upon reaching the Manila Cathedral, there will be a short program followed by a Mass, presided over by Cardinal Jose Advincula.
Last year, the "Walk for Life" was held from Welcome Rotonda in Quezon City to the University of Santo Tomas in Manila.
The "Walk for Life," spearheaded by the Catholic Bishops' Conference of the Philippines Episcopal Commission on the Laity, was first held in 2017 in response to the drug war killings in the country, as well as the proposed restoration of the death penalty.
Since then, it has become an annual event aimed at promoting the need to protect human life and the family from threats such as abortion, divorce, and same-sex unions.